Paraffin embedding and immunocytochemistry
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| From: | "LOVE, JOHN E. (JSC-SD)" <john.e.love1@jsc.nasa.gov> |
| To: | "'histonet@pathology.swmed.edu'" <histonet@pathology.swmed.edu> |
| Reply-To: | |
| Date: | Tue, 3 Aug 1999 14:04:53 -0500 |
| Content-Type: | |
Considering that paraffin embedding of tissues involves temperatures of 60
degrees C and deparaffinization protocols employ xylenes, I am afraid that
those procedures may result in denatured epitopes and shrunken cells that
could compromise immunocytochemical results.
I am interested in information regarding the effect of paraffin embedding
conditions on immunocytochemistry. Thanks for your help,
John
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