Paraffin embedding and immunocytochemistry

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From:"LOVE, JOHN E. (JSC-SD)" <>
To:"''" <>
Date:Tue, 3 Aug 1999 14:04:53 -0500

Considering that paraffin embedding of tissues involves temperatures of 60
degrees C and deparaffinization protocols employ xylenes, I am afraid that
those procedures may result in denatured epitopes and shrunken cells that
could compromise immunocytochemical results.  
I am interested in information regarding the effect of paraffin embedding
conditions on immunocytochemistry.  Thanks for your help,

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