Need help

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Date:Sun, 15 Aug 1999 17:51:24 EDT
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Hello Histo people,

We have Vashaw autostainer. We are having problem with uneven staining 
especially with our skins cases. We mount at least 6 or less frames on a 
slide. One of our pathologist is giving us a fit about the uneven staining. 
He may have a slide that is stain OK but one frame will be totally light with 
very little staining. We have tried everything we know. Decrease the acid 
alcohol that we use, longer time in the hema.We dry them in an oven for 10 
min. and then on the stainer for ten min. They were lifting at one time. Any 
help would be great.

Rita Riddle
Lexington Medical Center
West Columbia, SC

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