NSH membership ???

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From:"Greer, Bonnie" <Bonnie.Greer@stjude.org>
To:"'HistoNet@pathology.swmed.edu'" <HistoNet@pathology.swmed.edu>
Date:Fri, 6 Aug 1999 13:49:04 -0500
Content-Type:text/plain; charset="iso-8859-1"

Would someone please send me the address and form to join.......Thanks
Bonnie G.Greer		
ARC Histotechnologist
St. Jude Children`s Research Hospital
332 N. Lauderdale St.
Memphis, TN 38104
Fax 901-495-3112

	-----Original Message-----
	From:	Bryan Llewellyn [SMTP:bryand@netbistro.com]
	Sent:	Friday, August 06, 1999 1:10 PM
	To:	'Histonet'
	Subject:	Re: Paraffin Oil Red O staining

	I have occasionally done this.  It is a relatively well established
	of demonstrating lipofuscins, particularly the more fatty kind.
Yes, they
	do survive treatment with xylene in paraffin processing.

	I use the method with oil red O dissolved in propylene glycol and
stain for
	at least an hour.  You MUST mount with an aqueous mountant though,
or the
	dye is extracted.

	Bryan Llewellyn

	----- Original Message -----
	From: Histo-Scientific Research Laboratory <histosci@shentel.net>
	To: Linda McGraf <HistoNet@pathology.swmed.edu>
	Sent: August 6, 1999 6:16 AM
	Subject: Paraffin Oil Red O staining

	> Dear Histonetters,
	> Has anyone ever done an Oil Red O stain on paraffin embedded
tissues?  I
	> wondering why anyone would do frozens on fatty tissues if the
	> in tact when they where processed, and paraffin embedded?  I would
love to
	> know your results if you have done this procedure.
	> Thanks,
	> Beth Poole
	> Histo-Scientific Research Labs.
	> (540)856-2222
	> histosci@shentel.net
	> P.O. Box 30
	> 107 Killmon Road
	> Basye, VA  22810

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