More homemade humidity chambers & DAB filtering

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From:C & J Haley <>
To:HistoNet Server <>
Date:Fri, 13 Aug 1999 23:02:39 -0500
Content-Type:text/plain; charset=us-ascii

Several years ago we purchased a couple of humidity chambers from
Shandon/Lipshaw.  They were designed to hold about 40+  slides.  They
came with plastic shelves..  Of course we had to modify them.  We take
metal slide trays that would hold 20 slides.  We bundle up 7 slides and
tape them together.  Place a "bundle" in each corner position of the
slide tray. (Each tray will now hold 16 slides.) You can stack these on
top of each other in whatever little container you desire.  By using the
Shandon chamber and this method, we can get more than 100 slides in one
box.  We wet paper towels or gauze and put them to the side corners of
each box.  One of our pathologists who enjoys doing her own little IHC
runs didn't want to shell out the money for one of these chambers.  She
went to an office supply store and bought an art supply box that holds
about 7 of these stacked slide trays for $5.
Needless to say, we now recommend the cheaper chambers to visiting

Gayle, we don't filter our DAB.  We buy a metal enhanced DAB kit  from
Pierce.  The DAB/Nickel/Cobalt  comes in a liquid form and you dilute it
with their peroxide solution.  None of the Sigma DAB tablet/isopac silt.
It looks great!

Hope this helps.

Jane Haley
Barnes-Jewish Hospital
St. Louis, MO

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