Dear colleagues,

I would like to do immunohistochemistry on 1 micron LR white sections before
doing it on thin sections for EM. 

1)	How can I prevent sections from wrinkling? I don't want to use heat
to preserve antigenicity.

2)	Without using heat how can I attach them to slides so that they do
not fall off during the immuno. I have already tried to air-dry them on
Superfrost slides but they easily fall off after few washes.

Thanking you in advance for your help,

Lilith

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Lilith  Ohannessian-Barry
NRC, IBS,  
Ottawa, Ont. K1A  0R6
CANADA
Tel;613-993-6460
Fax;613-941-4475
e-mail; lilith.barry@nrc.ca <mailto:lilith.barry@nrc.ca>