Hi Histonetters,

   This is a slight variation of the question I asked before.
I'm working with a couple of researchers who are doing in-situ on whole
frog embryos and then processing them through alcohols and xylene-paraplast
mixes and finally overnight in paraplast.  They are complaining that the
tissue is very difficult to cut (alot of folds and wrinkles) and the tissue
is somewhat brittle.  I was wondering if there was anyone who had any
experience with this type of preparation and could offer some advice.  My
initial advice was cutting there time down in paraplast to a maximum of 2
hrs. instead of overnight.  Someone else also suggested they were getting
creases in their sections due to poor infiltration and recommended using a
vacuum oven for the paraplast stage.

Any suggestions would be much appreciated!

								Thanks,
								Frank
.....................................................................
: Frank Walmsley, B.S.          Dept. of Cell Biology & Anatomy     :
: Research Specialist           University of Arizona               :
: (office:  AHSC 4212)          P.O. Box 245044                     :
: (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :
: (FAX:  520-626-2097)	        (email:  frankw@u.arizona.edu)      :
:...................................................................:
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