Formaldehyde overfixation artifact in immunocytochemistry

<< Previous Message | Next Message >>
From:"LOVE, JOHN E. (JSC-SD)" <>
To:"''" <>
Date:Tue, 3 Aug 1999 13:36:19 -0500

Considering that excessive times in aldehyde fixatives may result in
overfixation artifact during immunocytochemistry (e.g. epitope masking,
increased background staining), I would appreciate input regarding
recommended times for fixation of early embryos or cell cultures in buffered
10% formalin (4% formaldehyde).  Thanks for your help,

<< Previous Message | Next Message >>