Formaldehyde overfixation artifact in immunocytochemistry
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| From: | "LOVE, JOHN E. (JSC-SD)" <john.e.love1@jsc.nasa.gov> |
| To: | "'histonet@pathology.swmed.edu'" <histonet@pathology.swmed.edu> |
| Reply-To: | |
| Date: | Tue, 3 Aug 1999 13:36:19 -0500 |
| Content-Type: | |
Considering that excessive times in aldehyde fixatives may result in
overfixation artifact during immunocytochemistry (e.g. epitope masking,
increased background staining), I would appreciate input regarding
recommended times for fixation of early embryos or cell cultures in buffered
10% formalin (4% formaldehyde). Thanks for your help,
John
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