Hi all,

I am trying to cryosection canine synovium and am having difficulty.  The 
section comes up with holes where the tissue should be.  I believe this is 
due to the amount of fat in the tissue.  Does anyone have advice on how to 
handle this type of tissue?  The cryostat I am using is set at 25 degrees, 
and the sections are 8 microns. 

Thanks for your help,

Forward response to llafranco@ucdavis.edu