CD4

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From:"ANN MARUSKA" <amarusk1@FAIRVIEW.ORG>
To:<histonet@pathology.swmed.edu>
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Date:Wed, 11 Aug 1999 08:14:20 -0600
Content-Type:text/plain; charset=US-ASCII

Help!
I have been trying to work up CD4, Ventana predilute(Novacastra) clone 1F6 on the NexES with NO success.  I have tried a steam retrieval for varying times with EDTA bufffer (ph8.0) using tonsil fixed in both NBF and B5.  I have tried both the basic DAB and Enhanced kits with and without amplification.  I even thought perhaps my first antibody was not good and have tried it with a second predilute.
Any help or suggestions would be most appreciated!
Ann Maruska
amarusk1@fairview.org 
Fairview-University Med Ctr
Mpls, MN
612-672-4005




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