CD31, pecam
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| From: | "Coskran, Timothy M" <timothy_m_coskran@groton.pfizer.com> |
| To: | "'Histonet@pathology.swmed.edu'" <Histonet@pathology.swmed.edu> |
| Reply-To: | |
| Date: | Thu, 12 Aug 1999 08:16:55 -0400 |
| Content-Type: | text/plain; charset="iso-8859-1" |
Jill,
We use Pharmingen's CD31, which they recommend that the antibody be stored
at 4C for up to one year. It has worked very well for us on
paraformaldehyde fixed tissue with a 20-30 minute pretreatment in tyrpsin.
We've also found that alcohol fixed tissue gives a great signal without any
antigen retrieval steps. As far as antibody storage and shelf-life, Gayle
Callis provided an excellent answer.
Good Luck
Tim Coskran
Pfizer
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