CD31, pecam

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From:"Coskran, Timothy M" <>
To:"''" <>
Date:Thu, 12 Aug 1999 08:16:55 -0400
Content-Type:text/plain; charset="iso-8859-1"


We use Pharmingen's CD31, which they recommend that the antibody be stored
at 4C for up to one year.  It has worked very well for us on
paraformaldehyde fixed tissue with a 20-30 minute pretreatment in tyrpsin.
We've also found that alcohol fixed tissue gives a great signal without any
antigen retrieval steps.  As far as antibody storage and shelf-life, Gayle
Callis provided an excellent answer.

Good Luck

Tim Coskran

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