Bone orientation

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From:Linda Jenkins <jlinda@ces.clemson.edu>
To:histonet@pathology.swmed.edu
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Date:Fri, 6 Aug 1999 17:35:04 -0400
Content-Type:text/plain; charset="us-ascii"

For :Johanna-
	One easy method for marking orientation is to cut a "V"-notch in
the bone with a band saw (good with larger bones) or my personal favorite
mini-power tool - the Dremel tool(good with smaller bones).  I, too, have
found that the India inks, tattoo dyes, etc. cannot handle all the
solutions involved in MMA processing.  Are you embedding cross sections of
long bone?  In order to delineate anterior from posterior, I blot dry the
portion of bone and superglue an applicator stick to the posterior section
and then embed in MMA.   Wood and bone have a lot of similar cutting
properties and it sections easily with the attached applicator stick.
Also, if you leave a small portion of the periosteum with attached muscle
you can zip a suture in the desired location.  Cuts well.
	Good luck,
	Linda
*********************************
Linda Jenkins, HT
Clemson University
Department of Bioengineering
Clemson, SC
**********************************



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