Autocyte immunohistochemistry

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Date:Fri, 13 Aug 1999 19:05:40 EDT
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Out cytopathologist is complaining mildly about background staining on smears 
prepared on our new cytology "Autocyte" instrument. When these smears are 
stained on our Ventana immunohistochemistry stainer, some neutrophils and 
polymorphs take up the DAB. We think the special fixative used with the 
Autocyte is to blame but are not really sure. The Autocyte company is not too 
forthcoming with information.  Has anyone else experienced this problem or 
know how to correct it? Any suggestions will be appreciated.

Michael Titford
Pathology Department
USA Medical Center
Mobile AL 36617

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