Histonetters,

I need your help.  While doing a VERY important immunohistochemical (IHC) run
and trying to teach three new people about IHC at the same time- I did the MOST
STUPID thing I've done in years.  The IHC run had two different chromagens - DAB
and AEC.  I know better (Murphy's Law) but I was in a big hurry plus overworked,
I threw both sets of slides into alcohol after the chromogen step.  Needless to
say, the AEC set of slides were ruined.  My new employees wanted to know why
they were ruined, and if there is a possible way to save them.  Besides just
saying "Because" or AEC is water soluble - what is a good explanation?  Is there
any possible way of saving the slides?  (again, the slides were/are an
important, rare tissue type)

Nancy Cardwell
Research Assistant III
Vanderbilt University Medical Center
Nashville, Tennessee