Re: [Histonet] bone marrow decalcification

From:"Joe Nocito"

Biocare Medical has ionic beads that attracts the calcium out from the 
specimen. You can use it up to 20 blocks, is really inexpensive and does 
improve your immunos.

----- Original Message ----- 
From: "Weems, Joyce" 
To: "Ryan, John P." ; 
Sent: Friday, August 22, 2008 3:53 PM
Subject: RE: [Histonet] bone marrow decalcification

B-Plus fixative from BBC Biochemical works well for us.

Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax

-----Original Message-----
[] On Behalf Of Ryan,
John P.
Sent: Friday, August 22, 2008 4:41 PM
Subject: [Histonet] bone marrow decalcification

What method do you utilize for the decalcification of bone marrow
specimens that does not impair immunohistochemical staining? We have
noted that our immunos (not surprisingly) stain less intensely on
decalcified bone marrow than on non-decalcified tissue and would like to
minimize this effect.

John Ryan
St. Luke's Episcopal Hospital

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