Re: [Histonet] bone marrow decalcification

From:"Mark Tarango"

Hi John,

Are you sure that the BM biopsies are completely fixed before going
into decal?  No pinkish or reddish-colored cores should be put into

I'd look into the fixation time before decalcification.  If you can
increase this, it might be all that you need to do.


On 8/22/08, Ryan, John P.  wrote:
> What method do you utilize for the decalcification of bone marrow specimens that does not impair immunohistochemical staining? We have noted that our immunos (not surprisingly) stain less intensely on decalcified bone marrow than on non-decalcified tissue and would like to minimize this effect.
> John Ryan
> St. Luke's Episcopal Hospital
> 832-355-2643
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