RE: [Histonet] fibrin gel to paraffin

From:"Patsy Ruegg"

I would wrap them in paper (perm curl paper) and process as usual on a
tissue processor, I use a shorter than usual schedule for animal tissue and
things like this, 20 min in each change of alcohols, xylene and paraffins.
Embed on edge to get a cross section of all the layers.

-----Original Message-----
[] On Behalf Of Molinari,
Sent: Thursday, August 21, 2008 5:44 AM
Subject: [Histonet] fibrin gel to paraffin


I have some "outgrowths" that are in a modified fibrin gel. They vary in
size from.1 to 1mm thick. The gel is porous and optically clear for the
most part. They have been fixed in 4% paraformaldehyde. They were done
in well plates or chamber slides.

 The researchers want them processed for paraffin. I have no experience
with this technique and would be grateful for any suggestions.

Thank you.


Betsy Molinari   HT(ASCP)

Texas Heart Institute 

Cardiovascular Pathology

6770 Bertner Ave.

MC 1-283

Houston, TX 77030


832-355-6812 (fax)


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