RE: [Histonet] bone marrow decalcification. .

From:"Henry, Charlene"

We use B+ fixative and EDTA/Formalin to decalcify BM Bxs. and they works great for IHC and even ISH. Many times the marker is more intense on the EDTA decalcified tissues than just formalin fixed tissues. It varies from antibody to antibody.

Charlene Henry HT (ASCP), QIHC
Anatomic Pathology Section Head
Department of Pathology
St. Jude Children's Research Hospital
fax 901-495-3100

-----Original Message-----
From: [] On Behalf Of Weems, Joyce
Sent: Friday, August 22, 2008 3:54 PM
To: Ryan, John P.;
Subject: RE: [Histonet] bone marrow decalcification. .

B-Plus fixative from BBC Biochemical works well for us.

Joyce Weems
Pathology Manager
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax

-----Original Message-----
[] On Behalf Of Ryan, John P.
Sent: Friday, August 22, 2008 4:41 PM
Subject: [Histonet] bone marrow decalcification

What method do you utilize for the decalcification of bone marrow specimens that does not impair immunohistochemical staining? We have noted that our immunos (not surprisingly) stain less intensely on decalcified bone marrow than on non-decalcified tissue and would like to minimize this effect.

John Ryan
St. Luke's Episcopal Hospital

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