RE: [Histonet] bone marrow decalcification

From:"Weems, Joyce"

B-Plus fixative from BBC Biochemical works well for us.

Joyce Weems
Pathology Manager
Saint Joseph's Hospital 
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax

-----Original Message-----
[] On Behalf Of Ryan,
John P.
Sent: Friday, August 22, 2008 4:41 PM
Subject: [Histonet] bone marrow decalcification

What method do you utilize for the decalcification of bone marrow
specimens that does not impair immunohistochemical staining? We have
noted that our immunos (not surprisingly) stain less intensely on
decalcified bone marrow than on non-decalcified tissue and would like to
minimize this effect.

John Ryan
St. Luke's Episcopal Hospital

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