RE: [Histonet] DAB: old-fashioned style!

From:"Tony Henwood"

Try this

DAB Reagent:
1.      Take 40ml Tris Buffer-Wash  and add 6mg of DAB (3,3, Diaminobenzidine Tetrahydrochloride Grade II (Sigma D5637)), mix.
2.      Add 50ěl of hydrogen peroxide, mix.
3.      The solution is ready to use.


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
Tel: 612 9845 3306
Fax: 612 9845 3318
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145

-----Original Message-----
From: [] On Behalf Of Kim Merriam
Sent: Saturday, 30 August 2008 3:07 AM
To: Histonet
Subject: [Histonet] DAB: old-fashioned style!

Hi All,

It's been many moons since I had to make DAB by hand and I have lost my recipe.  Can anyone let me know how to make DAB from powder and H2O2?

 Kim Merriam, MA, HT(ASCP)QIHC
Cambridge, MA

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