I have successfully done X-Gal on PFA fixed paraffin embedded specimens. The key is to not use xylene in processing or deparaffinization/clearing step.
I used XS3 from StatLab, but probablt any substitute would work.
Paula Pierce, BS, HTL(ASCP)HT
Excalibur Pathology, Inc.
631 N Broadway Ave.
Moore, OK 73160
405-759-3953
contact@excaliburpathology.com

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