I'm at home without my notes, but I remember John Kiernan
saying somewhere (Histonet, the Book?) that DAB dissolves
better in pure water. Since then that's what I've done -
dissolve in deionized water at 1mg/ml, vortex thoroughly, then
bring up to rxn volume in Tris, pH 7.6. Works a treat!
I stain thick (40um) sections, so I also like to preincubate
in my DAB solution plus any intensifiers for 8-10 min to allow
them to penetrate, then start the reaction by adding the
peroxide later - it's a tiny molecule and gets everywhere very
David A. Wright, PhD
Section of Neurosurgery
University of Chicago
Histonet Digest, Vol 57, Issue 41: Message: 2
Date: Fri, 29 Aug 2008 10:06:55 -0700 (PDT)
From: Kim Merriam
Subject: [Histonet] DAB: old-fashioned style!
It's been many moons since I had to make DAB by hand and I
have lost my recipe. Can anyone let me know how to make DAB
from powder and H2O2?
�Kim Merriam, MA, HT(ASCP)QIHC