Re: [Histonet] species control blocks

From:Rene J Buesa

During prolonged storage the epitope oxidizes and weaken. The weakening rate vary with the epitope, some are quite resistant and others weaken in 1 or 2 weeks. The rule of thumb is to cut enough controls that will last one week. It is better to cut both case and control of those weakening fast, that to lose all the reagents using one control already oxidized.
René J.

--- On Wed, 8/13/08, Perry, Margaret  wrote:

From: Perry, Margaret 
Subject: [Histonet] species control blocks
Date: Wednesday, August 13, 2008, 12:23 PM

I have a question for the people who do animal diagnostic work.  How
long do you keep paraffin species control blocks for such tests as
chromogranin a, CD3, CD79a, S100a, cytokeratin etc.  Have you noticed a
decline in antigenicity after a period of time?  How long is it before
you notice a decline?



Margaret Perry HT (ASCP)

IHC Lab Manager Veterinary Science

Animal Disease Research and Diagnostic Lab

South Dakota State University

Box 2175 North Campus Drive

Brookings SD 57007


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