Re: [Histonet] get back eosin stain?

From:"Mark Tarango"

Hi Yak-Nam,

The loss of eosinophilia is probably because the cells were cultured.
I'd suggest trying a  PAS and it might look more like what you'd like.


On 8/17/08, Yak-Nam Wang  wrote:
> Hello,
> I have a colleague with a staining problem. He has cells grown on polymeric
> scaffolds. He can not embedd in paraffin due to a mismatch in the processing
> and his scaffold material; he has thus embedded in OCT.
> This is his procedure:
> Fix cell seeded scaffold in formalin (these are delicate samples thus, fixing before embedding)
> Cryoprotect in grade sucrose to 30% sucrose
> Embed in OCT
> Section at 6um
> General H&E staining-using 1 min for Hematox and 1.5 min for Eosin (following
> one of Richard Allan suggested protocols).
> Problem: there is no eosin staining. The cells are just purple, there is no pink at all. We are not sure why this is. Is this caused by fixation?
> Does someone have any suggestions on how he can get the classic H&E stain look for these samples? He has tried fresh solutions as well as running other tissue (unfixed) through. It doesn't seem to be the solutions used.
> Any suggestions would be appreciated. Thank you
> Yak-Nam
> Reserach Associate
> University of Washington
> Box 355640
> Seattle, WA 98195
> Tel.:
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