Even if everything as you say is "tight", check the feeding mechanism (the connection screw/vertical advance of the block holder).
René J.

--- On Thu, 8/14/08, Dan Zadory  wrote:

From: Dan Zadory 
Subject: [Histonet] Sliding Microtome Taking Random Thicker Sections
To: histonet@lists.utsouthwestern.edu
Date: Thursday, August 14, 2008, 1:45 PM

Hello,
 
We have recently purchased an HM430 MICROM Sliding Microtome along with
the KS 34 Fast Freezing Unit. Recently, I have tried sectioning frozen
mouse brains at 40 microns and noticed after several cuts the Microtome
will take a random thicker section (I would have to say approximately
150-200 microns thick). I am unsure as to why it keeps doing this. The
knife is in the right position and all parts have been tightened. In
addition, I have the Fast Freezing Unit set to keep the stage at a
constant -22C. We placed a dehumidifier in the room since we thought the
humidity may play a role...but so far no luck. Any suggestions?
 
  
Daniel Zadory


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