Hi Melissa:

    Tissue that thick must be sliced into 3-5 mm slices to get adequate 
fixation in the center. While an alcohol-based fixative such as 
formalin-alcohol-acetic acid will penetrate faster than buffered 
formalin, your tissue is too thick for adequate fixation in the center 
without slicing it into smaller pieces.

Geoff

Hamilton, Melissa wrote:
> I am searching for a protocol for fixing mouse tumors (subcutaneously or
> orthotopically grown) that are approx 2 to 2.5 cm in diameter.  I am
> having issues with penetration throughout the tissue.  Thanks.
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>   


-- 
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583 
mcauliff@umdnj.edu
**********************************************



_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet