Re: [Histonet] Microwave fixation of whole organs

From:Rene J Buesa



Fixing whole organs in the microwave is the wrong practice. Any organ heated above 50ºC will determine that the proteins will be HEAT COAGULATED and that is absolutely other thing to fixing.
In the irresponsible quest for speed, by yielding to administrative pressures to discharge patients sooner than required and cut costs, we sometimes are ruining the tissues we have to work with later on.
Very bad practice!
René J.

--- On Fri, 8/15/08, Tracy Cloyd  wrote:

From: Tracy Cloyd 
Subject: [Histonet] Microwave fixation of whole organs
To: histonet@lists.utsouthwestern.edu
Date: Friday, August 15, 2008, 9:11 AM

Hello all,

Is anyone fixing whole organs (prostates, placentas) in the microwave?  
If so, what is your procedure?  I have heard of fixing a whole prostate 
for 5 minutes at 65°.  Seems like a short time.
 Thoughts?

-- 
Regards,

Tracy





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