I was not be able to get good quality sections from bone
specimens, until begann to use next things:
1- determination endpoint of decalcification
2- isopropanol as dehydratant
3- mineral oil as antemedium before parafiin infiltration.
In my personal experience only these things had great
improvement for quality of my bone sections.
All rest things have had minimal importance for me.
I must use manual processing, because have not any processor.
> Date: Tue, 5 Aug 2008 20:07:50 EDT
> From: BERGERJL@aol.com
> Subject: [Histonet] Horse bones
> To: firstname.lastname@example.org
> Content-Type: text/plain; charset="US-ASCII"
> We are trying to section pieces of horse femurs,
> fetlock and carpal joints.
> They have been decalcified with either HCl decal or
> formic acid decal.
> Before routine processing the bones appear to be
> decalcified, but at sectioning
> they are very hard and brittle (even after surface
> decal) and chip out of the
> paraffin block.
> Any suggestions would be helpful. A pathologist
> suggested a soap soaking
> solution but could not remember the name of the soap.
> Help and thank you,
> R. Berger, HT
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