Re: [Histonet] Alternatives to Weigert's Iron Hematoxylin for Mucin and Trichrom e Staining

From:"Mark Tarango"



Hi Tanisha, remember that 7211 hematoxylin is red (but turns blue when
blued).

You are using Weigert's Iron Hematoxylin because it's grey/black in color
and can be seen when there is a lot of color in a stain.  When looking for a
substitute like this, your best bet will be to stick to the same color.

Mark


On 8/8/08, McKnight, Tanisha  wrote:
>
> Hello All:
>
> Can anyone suggest an alternative to Weigert's Iron Hematoxylin for nuclei
> staining with Mucin and Trichrome protocols? I have some standard
> hematoxylin (7211) that I will be using for my routine staining. However,
> with the shortage, Weigerts is on backorder until October.
>
>
> Tanisha McKnight, HT(ASCP)
> AP-Histology/Specimen Management
> Covance CLS, Indianapolis
>
>
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