RE: [Histonet] uneven ihc staining

From:"William Connor"

If on an autostainer, it could also be the 'probe', which is what tech
support for our autostainer keeps saying...though not likely the case on

We are in FL and have noticed for the past couple of years inconsistent
staining on DUPLICATE slides has been a problem especially when the temp
rises and the humidity increases around June.  We are currently in the
process of observing this possibility and trying to regulate...

-----Original Message-----
[] On Behalf Of Bernice
Sent: Friday, August 08, 2008 2:48 PM
To:;; 'richard shook'
Subject: RE: [Histonet] uneven ihc staining

Could also be a sensor issue on an autostainer or if the unit has been moved
recently and not properly levelled.

Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611

-----Original Message-----
[] On Behalf Of Rene J Buesa
Sent: Friday, August 08, 2008 9:30 AM
To:; richard shook
Subject: Re: [Histonet] uneven ihc staining

Most times this effect is caused by uneven dewaxing of the slides.You should
have a very strict protocol for exchanging the dewaxing reagents.
René J.

--- On Fri, 8/8/08, richard shook  wrote:

From: richard shook 
Subject: [Histonet] uneven ihc staining
Date: Friday, August 8, 2008, 8:50 AM

Im looking for help we are having uneven staining on some (not all) ihc
we have looked into several different possoble reasons with no luck ,this
happens a couple times every week ao so any suggestion would be great 


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