RE: [Histonet] uneven ihc staining

From:"Bernice Frederick"

Could also be a sensor issue on an autostainer or if the unit has been moved
recently and not properly levelled.

Bernice Frederick HTL (ASCP)
Northwestern University
Pathology Core Facility
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611

-----Original Message-----
[] On Behalf Of Rene J Buesa
Sent: Friday, August 08, 2008 9:30 AM
To:; richard shook
Subject: Re: [Histonet] uneven ihc staining

Most times this effect is caused by uneven dewaxing of the slides.You should
have a very strict protocol for exchanging the dewaxing reagents.
René J.

--- On Fri, 8/8/08, richard shook  wrote:

From: richard shook 
Subject: [Histonet] uneven ihc staining
Date: Friday, August 8, 2008, 8:50 AM

Im looking for help we are having uneven staining on some (not all) ihc
we have looked into several different possoble reasons with no luck ,this
happens a couple times every week ao so any suggestion would be great 


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