RE: [Histonet] re-staining an IHC slide for H&E

From:"Dawson, Glen"


I would suggest doubling the Hematoxilyn time if the negative went through an enzyme retrieval as this would inhibit the basophillic staining for your H&E.  Otherwise it is actually just as easy as Rene states below.

Good Luck,
Glen Dawson

-----Original Message-----
[]On Behalf Of Rene J
Sent: Wednesday, August 13, 2008 10:27 AM
To:; Price, Tiffany
Subject: Re: [Histonet] re-staining an IHC slide for H&E

Just take the coverslip off, hydrate and stain as usual.
René J.

--- On Wed, 8/13/08, Price, Tiffany  wrote:

From: Price, Tiffany 
Subject: [Histonet] re-staining an IHC slide for H&E
Date: Wednesday, August 13, 2008, 9:51 AM

I have been asked to take a negative control slide and re-stain it for
H&E. Does anyone have a protocol for this?
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