We filter ours thru a wet blue sponge, place another sponge on top and
process as usual. After processing we place the sponges in paraffin and
place on the cold plate for a few seconds(using a tamper), pull the sponge
off slowly and melt the contents again (pulling out what is trapped in the
sponge). This process will capture any tissue present.
Cheryl Miller HT (ASCP)
Histology Supervisor
Physicians Laboratory,P.C.
Omaha, Ne.
402 738 5052
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Debra D.
Nannenga
Sent: Saturday, August 09, 2008 10:07 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] flitering methods
Hello Histonetters,
I was wondering what everyone was using for filtering and processing
ECC's, EMC's and the like. We have always used lens papers cut to an
appropriate size. We are now having difficulty getting them to even filter,
the liquid and tissue just sit on the paper and the liquid does not drain
away. I am wondering if this is a new and improved lens paper and that is
why it is not working now.
Any suggestioons you are willing to share would be appreciated.
Debbie Nanennga, HTL,QIHC
InCyte Pathology
13103 E. Mansfield
Spokane Valley, WA 99216
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