RE: [Histonet] Substitute Hematoxylin Dyes

From:"Caldwell, Lia"

We tested the Phoenix Blue with our Pathologists and the general consensus was that it was far inferior to the H/E stain.  Our pathologists are used to a regressive H/E and Phoenix Blue can only be run progressively.  The pathologists seemed troubled with the nuclear detail provided by Phx Blue particularly in Gi biopsies (which is the majority of our volumes).  We will be testing Tango next - I will keep you posted if you are interested.  
Lia M. Caldwell HT (ASCP)
Histology Supervisor
=0AOro Valley Pathology Dept.
phone: (520) 901-3914
"be yourself - everyone else is already taken." -unknown


=0AFrom: on behalf of Dana Settembre
Sent: Thu 8/14/2008 8:16 AM
To:; Vivian Deanna Snider
Subject: Re: [Histonet] Substitute Hematoxylin Dyes

Hello Deanna,
I can only tell you about how Phoenix Blue worked as a counterstain
for immunos and it does not turn blue in the ammonia.
It remained a lilac color which was difficult with the DAB.
If the DAB staining was light on the patient case it was tough.

Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA

>>> "Snider, Vivian Deanna"  08/14/08 11:00 AM
Dear Netters,

What are you substituting for Hematoxylin?  I have narrowed it down to
I want to research; The Tango Stain, from Anatech and Phoenix Blue
Nuclear Stain, ordered from Fisher.  Is anyone familiar with either of
these stains?  If so, what are the positive/negatives about each one?
Are the results as comparable to the actual Hematoxylin dye?  I have
searched the net for images of a completed stain, to no avail.  Any
information will be greatly appreciated .

Thanks in advance.=0D
Deanna Leslie HT ASCP

Lead Histotechnician

=0AHistology Research Laboratory

Shriners Hospital for Children=0D
Cincinnati, OH

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