RE: [Histonet] Perl's iron stain + IF together

From:"Liz Chlipala"


I'm not sure about IF but you can do perls with routine IHC with either DAB or AP.  If you are going to use DAB stain the IHC first then stain the perls.  If you are doing AP detection with fast red then stain with perls first and then perform the IHC.  


Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
P.O. Box 18592
Boulder, CO 80308
phone (303) 682-3949
fax (303) 682-9060
Ship to Address:
Premier Laboratory, LLC
1567 Skyway Drive
Unit E
Longmont, CO 80504

-----Original Message-----
From: [] On Behalf Of Kim Merriam
Sent: Friday, August 15, 2008 10:22 AM
To: Histonet
Subject: [Histonet] Perl's iron stain + IF together

Has anyone ever done a Perl's iron stain in conjunction with IF staining for CD markers?  I am not sure which I would need to do first or if one would mask the other.
Any thoughts?
 Kim Merriam, MA, HT(ASCP)
Cambridge, MA

Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>