RE: [Histonet] Freezing Haematoxylin

From:"Barnhart, Tammy"

Yep, if it freezes it's worthless.  From a North Dakota histotech.  We HAVE
to make our own.  Winter shipments of premaid hematoxylin always get too
cold and the staining is very erratic.

-----Original Message-----
[]On Behalf Of Rene J
Sent: Friday, August 15, 2008 12:08 PM
To: louise renton;; Weems, Joyce
Subject: RE: [Histonet] Freezing Haematoxylin

Ah, the things you learn when living in the "cold area" of the country!
René J.

--- On Fri, 8/15/08, Weems, Joyce  wrote:

From: Weems, Joyce 
Subject: RE: [Histonet] Freezing Haematoxylin
To: "louise renton" ,
Date: Friday, August 15, 2008, 12:07 PM

I don't think so... If hematoxylin freezes in shipping, it crystallizes
and must be replaced. My 2 cents worth... 

-----Original Message-----
[] On Behalf Of louise
Sent: Friday, August 15, 2008 3:41 AM
Subject: [Histonet] Freezing Haematoxylin

Dear all,

Here's a question to tickle your brain cells before the weekend

Can I freeze aliquots of Mayer's Haem (home made) for later use? Would
there be any negative effects on composition/staining etc???

Louise Renton
Bone Research Unit
University of the Witwatersrand
South Africa
"There are nights when the wolves are silent and only the moon
George Carlin
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