I have an intellectual question for you all. I have recently stained a
colon tumor with p53 from Novocastra and got clear areas of positive and
negative staining for no visible reason (i.e. Normal tissue vs. tumor
tissue). It looks like a fixation issue but in some areas there will be no
staining then a small group of cells (like an island) will stain. Iım not
sure how to check if this is a biological function or if this is caused by
something pre-analytical. Can anyone help?
Aprill Watanabe, B.S.
Research Associate
Integrated Cancer Genomics Division
Tissue Microarray Center (TMA)
Translational Genomics Research Institute (TGen)
main: 602-343-8822
Fax: 602-343-8840
awatanabe@tgen.org
www.tgen.org
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