I tried this in a much simple way.
After fixation and 30% sucrose, put the brain into 10% gelatin.
Then 37 degree for 1 h, with 4 'C for 20 min.
Then put the brain back to PFA for 3 h, followed with 48 h 30% sucrose.
Now you can go microtome.
发件人： Sarah Boyd
发送时间： 2008-08-05 23:25:41
主题： gelatin embedding
Hi! I have an embedding protocol, but I wouldn’t say it’s the greatest for our application, which are scaffolds. It may work for you though. The only issue I have with it is the histo staining. The gelatin picks up everything! Will you please let me know if you get another good one from someone else? I would be interested in trying another one. This protocol is written for use with scaffolds but you can modify it for your use.