[Histonet] Re: gelatin embedding


I tried this in a much simple way.

After fixation and 30% sucrose, put the brain into 10% gelatin.
Then 37 degree for 1 h, with 4 'C for 20 min.
Then put the brain back to PFA for 3 h, followed with 48 h 30% sucrose.
Now you can go microtome.



发件人: Sarah Boyd 
发送时间: 2008-08-05  23:25:41 
收件人: tifei@foxmail.com 
主题: gelatin embedding 
Hi!  I have an embedding protocol, but I wouldn’t say it’s the greatest for our application, which are scaffolds.  It may work for you though.  The only issue I have with it is the histo staining.  The gelatin picks up everything!  Will you please let me know if you get another good one from someone else?  I would be interested in trying another one.  This protocol is written for use with scaffolds but you can modify it for your use.

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