Hi Vikki
about a year ago we were testing a visualization system that probably came
from the 'same kitchen' as does your stuff. It also contained a 'post
primary block'. When you omitted the post primary while using rabbit
primaries, you had good staining, however, when you did the same while using
mouse primaries you've got nothing. In the old days, we used to call this
'post primary block' a secondary antibody: the polymer in the visualization
system tested was anti-rabbit only, so you had to 'turn' all your mouse
primaries into 'rabbits' by adding a rabbit-a-mouse secondary antibody. But,
of course, a visualization system that contains an extra-enhancing
intergalactic superduperwhatsoever post primary block sounds a lot better
than something that contains such a simple thing as a secondary antibody ...
If you repeat our experiment and may be run a few slides with mouse
primaries with and without 'post primary block' as well as with a
rabbit-a-mouse secondary of your choice (it may even be biotinylated, should
still work) you may be able to tell, whether your 'post primary block' has
the same function as did ours. Good luck!
Best regards
Andi Kappeler
Institute of Pathology, University of Bern, Switzerland
-----Ursprüngliche Nachricht-----
Von: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] Im Auftrag von Victoria
Baker
Gesendet: Freitag, 1. August 2008 20:13
An: Histo Net list server
Betreff: [Histonet] A question for BondMax users
Happy Friday everyone!
In the BondMax detection system there is a "post primary" step that contains
10% BSA in a Tris buffer. I've tested the protocol excluding this step and
did not have any staining.
I can't seem to get the answer I'm looking for from the company, so if
anyone can help me I'd really appreciate it.
Thanks
Vikki
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