John,Are you saying that IF stained slides can go through the normal dehydration/clearing process and be mounted in a xylene-based mounting medium?Kim Kim Merriam, MA, HT(ASCP)Cambridge, MA----- Original Message ----From: John Kiernan To: AGrobe2555@aol.comCc: histonet@lists.utsouthwestern.eduSent: Wednesday, August 29, 2007 1:31:52 AMSubject: Re: Re:[Histonet] IF-fading retardants=0AWhy do you "suspect you would kill your signal" by dehydrating, clearing and mounting a slide carrying a fluorescently labelled antibody? =0AAlcohol coagulates proteins on-the-spot, complete with any covalently bound fluorescent tags. This was established for lectin histochemistry 30 years ago, and has been well documented for fluorescently labelled antibodies in more recent years. The intensity of fluorescence emission may be less in DPX than in buffered glycerol, but who has done comparisons? A fairly recent study strongly favours alcohol dehydration, clearing and mounting in a non-flourescent recinous medium. for permanent immunofluorescence slides.John KiernanAnatomy, UWOLondon, Canada--- ----- Original Message -----From: AGrobe2555@aol.comDate: Monday, August 27, 2007 14:31Subject: Re:[Histonet] IF-fading retardantsTo: histonet@lists.utsouthwestern.edu> Carl,> I used to use "FluorSave" mounting medium from Calbiochem (Cat > # 345789). > This mounting medium "hardens" so the coverslip doesn't > move and you don't > have to seal around the edges with nail polish. It also > kept the signal from > fading. I still stored the slides horizontally at 4C > or -20C. I wouldn't > suggest try in to dehydrate through xylene and > coverslipping, as I suspect you > would kill your signal. > Albert> > =0A> Albert C. Grobe, PhD> International Heart Institute of Montana Foundation> Tissue Engineering Lab, Saint Patrick Hospital> > > =0A> > > ************************************** Get a sneak peek of the > all-new AOL at > http://discover.aol.com/memed/aolcom30tour> _______________________________________________> Histonet mailing list=0A> Histonet@lists.utsouthwestern.edu> http://lists.utsouthwestern.edu/mailman/listinfo/histonet> _______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet ____________________________________________________________________________________Need a vacation? Get great dealsto amazing places on Yahoo! Travel.http://travel.yahoo.com/
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