Re: [Histonet] working with vibratome sections

From:Phil McArdle

Hello Caroline:

This is from a vendor (EBS is a Vibratome dealer): I'm sure I can get an 
answer for you, but I'd need some specifics: what model Vibratome, what 
type of blade and holder, are you using refrigeration, and any other 
details however insignificant they may seem (e.g., fresh brain?). Brain, 
as I'm sure we're all painfully aware, is notoriously finicky and "the 
devil is in the details." For example, pediatric brain, being more 
hydrated, often requires different handling from mature brain.

Best regards,

Phil McArdle
Phil McArdle
Microwave Product Manager

Energy Beam Sciences, Inc.
29-B Kripes Rd.
East Granby, CT 06026

Tel:  800.992.9037 x 341
Mobile: 860.597.6796
Fax: 860.653.0422

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Caroline Bass wrote:
> Hey guys,
> I've been working with some vibratome sections of brain and could use 
> some advice.  I sectioned one brain and it came out ok.  The blade has a 
> tendency to hang on the last bit of tissue and flip it over producing 
> and section with a slight thickening at the end.  Despite this I've 
> gotten some good sections, but I'm having some problems processing 
> them.  Is it possible to simply immerse some in fix?  What is the best 
> way for handling this tissue?  I'd like to take some and dissect out the 
> brain regions I'm interested in and use the rest for histology.  
> Currently the sections are 300 microns.  Is this too think to coverslip?
> I've cut anther brain today and it went horribly, the brain just got 
> flattened, it seemed to get squished when the blade came along, I could 
> get a cut but it wasn't even, and I couldn't get any useful sections.  
> Any advice?  Are there any online resources for how to effectively use 
> the vibratome for brains.
> Thanks,
> Caroline
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