TEM or SEM?
Fully spread platelets can be done as whole mounts by either method.
SEM requires low kV accelerating voltages, less than 3 kV, 1.5 or
lower is better (platelets are basically invisible in the SEM at 10
kV and above, the beam goes right through them).
Ralph Albrecht et al in the 80s and 90s published several articles on
platelets using SEM and TEM. I'd look up his papers/book chapters.
>Does anyone have a procedure for preparing platelets for EM examination?
Ronnie Houston, MS, HT(ASCP)QIHC
Anatomic Pathology Manager
Columbus Children's Hospital
700 Children's Drive
Columbus, OH 43205
(614) 722 5465
Microscopy Facility Supervisor
024C Brooks Hall
Central Michigan University
Mt. Pleasant, MI 48859
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