If used above the expiration date it will cause what you describe. You will have to buy a new one of prepare your own with the following recipe:
  1- bovine serum--- 1 mL
  2- PBS buffer --- 99 mL
  3- Tween 20 ---- 0,05 mL
  4- sodium azide --- 100 mg
  Store at 4ºC
  René J.

Kimberly Tuttle  wrote:
  Can antibody diluent go bad, and cause non-specific staining? I have no idea how old it is and theres no expiration date.





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