Thanks to everyone who responded. Once again, the histonet proves to be an invaluable resource! KimKim Merriam, MA, HT(ASCP)Cambridge, MA----- Original Message ----From: Bernice Frederick To: Liz Chlipala ; Kim Merriam ; Histonet Sent: Monday, August 20, 2007 1:59:01 PMSubject: RE: [Histonet] IF staining of mast cells and fibroblastsMast cell tryptase works fine on human 1:8000.Bernice Frederick HTL (ASCP)Northwestern UniversityPathology Core Facility710 N Fairbanks CourtOlson 8-421Chicago,IL 60611312-503-3723-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of LizChlipalaSent: Monday, August 20, 2007 11:20 AMTo: Kim Merriam; HistonetSubject: RE: [Histonet] IF staining of mast cells and fibroblastsKimMast cell tryptase works in mice also, so I suspect it might work inother species. For fibroblasts there is a marker that works in rat itsmouse anti rat prolyl-4-hydroxylase beta (fibroblast marker) antibody6-9H6 (AF5110-1) from Acris. Liz =0AElizabeth A. Chlipala, BS, HTL(ASCP)QIHCManagerPremier Laboratory, LLCP.O. Box 18592Boulder, CO 80308phone (303) 735-5001fax (303) 735-3540liz@premierlab.comwww.premierlab.comShip to Address:Premier Laboratory, LLCUniversity of Colorado at BoulderMCDB, Room A3B40Boulder, CO 80309-----Original Message-----From: histonet-bounces@lists.utsouthwestern.edu[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of KimMerriamSent: Monday, August 20, 2007 9:46 AMTo: HistonetSubject: [Histonet] IF staining of mast cells and fibroblastsHello all,I was wondering if anyone knew of specific markers for mast cells andfibroblasts. I need markers for IF and IHC (not special stains) thatwill stain only mast cells and only fibroblasts. We have a few markersin mind, but we are not sure if they are specific only to those types ofcells and will not cross-react with some other cell type.Thanks in advance,KimKim Merriam, MA, HT(ASCP)=0ACambridge, MA ____________________________________________________________________________________Got a little couch potato? Check out fun summer activities for kids.http://search.yahoo.com/search?fr=oni_on_mail&p=summer+activities+for+kids&cs=bz_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonetNo virus found in this incoming message.Checked by AVG Free Edition. Version: 7.5.484 / Virus Database: 269.12.0/961 - Release Date:8/19/2007 7:27 AMNo virus found in this outgoing message.Checked by AVG Free Edition. Version: 7.5.484 / Virus Database: 269.12.0/961 - Release Date:8/19/2007 7:27 AM_______________________________________________Histonet mailing listHistonet@lists.utsouthwestern.eduhttp://lists.utsouthwestern.edu/mailman/listinfo/histonet=0A ____________________________________________________________________________________Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. http://mobile.yahoo.com/go?refer=1GNXIC
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