Re: [Histonet] IF staining of mast cells and fibroblasts

From:"Bryan Hewlett"


In which species?
For human mast cells in FPPE sections, we used Mast cell tryptase for IHC.


----- Original Message ----- 
From: "Kim Merriam" 
To: "Histonet" 
Sent: Monday, August 20, 2007 11:21 AM
Subject: [Histonet] IF staining of mast cells and fibroblasts

Hello all,

I was wondering if anyone knew of specific markers for mast cells and 
fibroblasts.  I need markers for IF and IHC (not special stains) that will 
stain only mast cells and only fibroblasts.  We have a few markers in mind, 
but we are not sure if they are specific only to those types of cells and 
will not cross-react with some other cell type.

Thanks in advance,


Kim Merriam, MA, HT(ASCP)
Cambridge, MA

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