Re:[Histonet] IF-fading retardants

I used to use "FluorSave" mounting medium from Calbiochem (Cat #  345789).  
This mounting medium "hardens" so the coverslip doesn't move  and you don't 
have to seal around the edges with nail polish.  It also kept  the signal from 
fading.  I still stored the slides horizontally at 4C or  -20C.  I wouldn't 
suggest try in to dehydrate through xylene and  coverslipping, as I suspect you 
would kill your signal.  

Albert C.  Grobe, PhD
International Heart Institute of Montana Foundation
Tissue  Engineering Lab, Saint Patrick  Hospital

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