Carl,
I used to use "FluorSave" mounting medium from Calbiochem (Cat # 345789).
This mounting medium "hardens" so the coverslip doesn't move and you don't
have to seal around the edges with nail polish. It also kept the signal from
fading. I still stored the slides horizontally at 4C or -20C. I wouldn't
suggest try in to dehydrate through xylene and coverslipping, as I suspect you
would kill your signal.
Albert
Albert C. Grobe, PhD
International Heart Institute of Montana Foundation
Tissue Engineering Lab, Saint Patrick Hospital
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