Re: [Histonet] HELP

From:mari.ann.mailhiot@leica-microsystems.com



HI Dorothy

It sounds like xylene is in the wax. The other possibility could be water.
Check the bottom of the paraffin tank and see if you have  droplets. If you
do then it is more than likely water.

Regards

Mari Ann Mailhiot BA HT ASCP
Application Specialist
Leica Technical Assistance Center
800 248 0123 x7267
847 236 3063 fax
mari.ann.mailhiot@leica-microsystems.com
www.leica-microsystems.com


                                                                           
             "Webb, Dorothy L"                                             
                                      histonet@lists.utsouthwestern.edu   
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             lists.utsouthwest                                     Subject 
             ern.edu                   [Histonet] HELP                     
                                                                           
                                                                           
             08/21/2007 12:19                                              
             PM                                                            
                                                                           
                                                                           



What would cause the tissues when taken out of the processor at the end
of the cycle to feel "slippery", almost greasy?  I was off and back
today and when the tech took the cassettes out of the processor, she
mentioned this and they are not cutting well at all.  Our processor was
just changed yesterday and as far as I can tell from smell, all reagents
are in order!  Where should I start?????  Thanks so much for answering
this so quickly..I am stymied!!

Dorothy Webb, HT (ASCP)
Histology Technical Supervisor
Regions Hospital, Pathology Department
640 Jackson Street, Saint Paul, MN 55101-2595
Phone: 651-254-2962
Fax: 651-254-2741
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