Re: [Histonet] Fw: Thiocarbohydrazide

From:John Kiernan

The answer to your question is simple.
Buy a major histochemistry book and read it. That means the last edition of Pearse. All three volumes will cost about as much as 3 ml of of a rather dilute primary antibody that might turn out to be be worthless. 
Books by Hayat (in all biomedical libraries) also have much to say about thiocarbohydrazide. This is a field in which you must know what you are doing. Following a procedure that you do not understand will not deliver meaningful results.
John Kiernan
Anatomy, UWO
London, Canada
> ----- Original Message ----- 
> From: Agustin Chertcoff 
> To: 
> Sent: Monday, August 13, 2007 5:47 PM
> Subject: Thiocarbohydrazide
> Hi Histoneters!
> I need a protocole for OTO Technics (Osmium tetroxide, 
> Thiocarbohydrazide) for Electron Microcoscopy, specially as 
> preparing the Thiocarbohidrazide solution and the correct 
> temperature 
> Thanks in advanced !
> Ht Agustin Victor Chertcoff
> Electron Microscopy Service
> National Institute of Microbiology C G Malbran
> Buenos Aires
> Argentina 
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