Re: [Histonet] APTS Coating of Microscope Slides

From:"Greg Dobbin"

Hi Jerry,
You'll have to decide for yourself if all the manual labour involved is
economical, but to answer your question: the silane imparts a positive
charge on the slide. There is is no coating per se that will change
color or otherwise deteriorate over time. The charge, however, can be
lost if the slides get wet while in storage (ie before use). Hope this

Greg Dobbin, R.T.
Chief Technologist, Histology Lab
Dept. of Laboratory Medicine,
Queen Elizabeth Hospital,
P.O. Box 6600
Charlottetown, PE    C1A 8T5
Phone: (902) 894-2337
Fax: (902) 894-2385

>>> JR R  8/28/2007 7:22 PM >>>

Back when I was a lad I coated all of my own slides using APTS, or
It worked pretty well at keeping sections on the slides.  We have been
using Superfrost Plus charged slides for a while now, but it is costing
us about $500.00 per month just in slides. 
Can anyone think of a reason I ought not to go back to just coating my
own slides?  Will the coated slides themselves change color over time,
interfere with my stains, etc?
I do mostly Movat Pentachrome stains and IHC.

Jerry L. Ricks
Research Scientist
U.W. Medicine at South Lake Union
Department of Pathology
Recharge--play some free games. Win cool prizes too!

Histonet mailing list

Statement of Confidentiality
This message (including attachments) may contain confidential or privileged information intended for a specific individual or organization.  If you have received this communication in error, please notify the sender immediately.  If you are not the intended recipient, you are not authorized to use, disclose, distribute, copy, print or rely on this email, and should promptly delete this email from your entire computer system.  

D?claration de confidentialit?
Le pr?sent message (y compris les annexes) peut contenir des renseignements confidentiels ayant pour objet une personne ou un organisme particulier. Si vous avez re?u la pr?sente communication par erreur, veuillez en informer l'exp?diteur imm?diatement. Si vous n'?tes pas le destinataire pr?vu, vous n'avez pas le droit d'utiliser, divulguer, distribuer, copier ou imprimer ce courriel ou encore de vous en servir, et vous devriez l'effacer compl?tement de votre syst?me informatique.

Histonet mailing list

<< Previous Message | Next Message >>