Re: [Histonet] APTS/TESPA coating, other slide coating

From:Rene J Buesa

Even if you dilute the albumen after filtering it (during preparation), you will have an increase in background noise.
  René J

Emily Sours  wrote:
  On this note, has anyone noticed that egg albumin-coated slides are better
than TESPA (3-aminopropyltriethoxysilane)
-coated slides for paraffin sectioning? We're using them for H&E and IHC. I
assume that egg-albumin would have more background in IHC, but I haven't
tried it yet.

Remember our war hysteria, when we called sauerkraut 'Liberty cabbage' and
somebody actually proposed calling German measles, 'Liberty
measles?'...Remember when the hick legislators in certain states, in
obedience to William Jennings Bryan, who learned his biology from his pious
old grandma, set up shop as scientific experts and made the whole world
laugh itself sick by forbidding the teaching of evolution?
--Sinclair Lewis, It Can't Happen Here, 1935
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