Try doing the IHC for eosinophils first and do a toluene blue for mast
cells as a counter stain.


Mark Adam Tarango HT(ASCP)

Histology & IHC Supervisor

Nevada Cancer Institute

One Breakthrough Way

Las Vegas, NV  89135

Direct Line (702) 822-5112

Mobile (702) 759-9229

Fax (702) 939-7663

  


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Moran
Elishmereni
Sent: Tuesday, August 28, 2007 2:34 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] staining mast cells and eosinophils

Hello,

I am very new at histology and immunohistochemistry, so please excuse
the
simplicity of my questions. I wish to stain parrafin-embedded slides
(murine/human skin and lung) for mast cells and eosinophils. That is- I
want
to be able to detect both cells on one slide. Is there anyway to stain
with
toluidine blue and also counterstain with another dye for eosinophils?
Alternatively- can i stain for toluidine blue to get mast cells, and
then do
(on the same slide) immunohistochemistry using an anti-MBP antibody to
detect eosinophils? I guess its more of a general question- can one use
a
simple dye AND immunohistochemistry (with antibodies) on the same slide,
or
is there interference? I would be very glad to receive advice and
protocols.

Many thanks,
Moran Elishmereni

Department of Pharmacology
School of Pharmacy, Faculty of Medicine
The Hebrew University of Jerusalem
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